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Spectrofluorimeter FluoroMax-4, HORIBA, Jobin Yvon

Spectrofluorimeter FluoroMax-4, HORIBA, Jobin Yvon

Spectrofluorimeter FluoroMax-4 HORIBA Jobin Yvon offers analytical speed and the ultimate sensitivity in fluorescence investigations. Fluorescence spectroscopy has assumed a major role in analysis, as for applicable compounds fluorescence gives high sensitivity and high specificity. High sensitivity results from a difference in wavelength between the exciting and fluorescence radiation. These results in a signal contrasted with essentially zero background. High specificity results from dependence on two spectra: the excitation and the emission spectrum. 3-D matrix scanning and contour-mapping provide a unique fingerprint that qualitatively identifies a compound. In florescence analysis, the amount of light emitted characteristically under suitable excitation is used as a measure of the concentration of the responsible species. Concentrations as low as fg/ml can be determined.

Applications:

  1. Estimation of subtle differences in protein tertiary structure by monitoring intrinsic tryptophan fluorescence of proteins
  2. Determination of binding constants of small molecules (such as polyphenols) to proteins by tryptophan fluorescence quenching method
  3. Investigation of of protein surface hydrophobicity (or small changes in tertiary structure influencing surface hydrophobicity) by monitoring of binding of hydrophobic fluorescent probe (such as ANS, 8-anilino-1-naphtalensulfonic acid )
  4. Monitoring of Maillard reaction by detection of fluorescent Maillard reaction products
  5. Highly sensitive determination of enzyme activity with fluorescent substrate
  6. Evaluation of interactions of biologically active coordinated compounds, such as platinum (IV) and ruthenium (II) complexes, with human serum albumin and calf thymus DNA
  7. Correlation of structure and features of natural and synthetic molecules and their metal complexes

Room No: 509

Contact person 1: Prof. dr Ljuba Mandić, lab 403, phone: 333 66 76

Characteristics:

  1. Excitation: Range 200-950nm
  2. Emission: Range 200-950nm
  3. Bandpass: 0-15nm, continuously adjustable from computer
  4. Integration time: From 1 ms to 160s
  5. Scan speed: 80 nm/s
  6. Water Raman signal: min. 400.000 cps minimum at 350 nm excitation, 397 nm emission, 5 nm bandpass, 1 s integration time
  7. Signal/Noise ratio: 3000/1
  8. Thermostated single cuvette holder. Designed for 1cm pathlength cuvettes. Maintains temperature from -20 to 80C

Publications reported results obtained with FluoroMax-4, HORIBA, Jobin Yvon:

  1. Stanic-Vucinic D, Prodic I, Apostolovic D, Nikolic M, Cirkovic Velickovic T: Structure and antioxidant activity of beta-lactoglobulin-glycoconjugates obtained by high-intensity ultrasound induced Maillard reaction in aqueous model systems and neutral conditions. Food Chemistry 2013;138: 590-599
  2. Stojadinovic M, Ognjenovic J, Radosavljevic J, Vesic J, Stanic-Vucinic D, Cirkovic Velickovic T: Binding affinity between dietary polyphenols and beta-lactoglobulin negatively correlates with the protein susceptibility to digestion and total antioxidant activity of formed complexes. Food Chemistry 2013;136:1263-1271
  3. Stanic-Vucinic D, Stojadinovic M, Atanaskovic-Markovic M, Ognjenovic J, Gronlund H, Van Hage M, Lannto R, Sancho A, Cirkovic Velickovic T: Structural changes and allergenic properties of beta-lactoglobulin upon exposure to high-intensity ultrasound. Molecular Nutrition & Food Research 2012, 56 (12): 1894-1905



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