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Rotor-Gene Q, Real-time PCR cycler and High Resolution Melt analyzer

Rotor-Gene Q (Qiagen, Germany) is a real-time nucleic acid amplification and detection system, which measures nucleic acid signals from amplified DNA using fluorescent detection. This world popular real-time PCR cycler and High Resolution Melt (HRM) analyzer, has recently been successfully installed and utilised at Faculty of Chemistry in Belgrade. It has optimal thermal and optical performance, due to rotary format and robust technology, requiring minimal maintenance. Rotor-Gene Q came with user-friendly software, GeneQuest (version 2.0.2.4), with advanced security features, that runs from a laptop computer (see photograph).

Rotor-Gene Q, Real-time PCR cycler and High Resolution Melt analyzer

Applications:

  1. Gene expression analysis
  2. Micro RNA detection
  3. Virus detection
  4. SNP genotyping
  5. High Resolution Melt analysis (HRM)

Characteristics:

  1. Optical System: It has 5 channels for optical detection (green, yellow,orange, red, crimson) plus HRM channel; Excitation sources: High energy light-emitting diodes; Detector: Photomultiplier; Acquisition time: 4 seconds
  2. Channel         Excitation (nm)         Detection (nm)
  3.   Green             470 +/- 10                  510 +/- 5
      Yellow             530 +/- 5                    557 +/- 5
      Orange           585 +/- 5                    610 +/- 5
      Red*               625 +/- 5                    660 +/- 10
      Crimson          680 +/- 5                    712 high pass
      HRM*              460 +/- 20                  510 +/- 5
  4. Thermal performance: Temperature range, 35C to 99C (95F to 210.2F); Temperature accuracy, +/-0.5C; Temperature resolution, +/-0.02C (smallest programmable increment); Temperature uniformity, +/-0.02C (standard deviation)
  5. Samples per run: Strip Tubes 0.1 ml - 72 samples/run; Tubes 0.2 ml - 36 samples/run
  6. Humidity: 10-75% (noncondensing)
  7. Operating temperature: 18-30C (64-86F)

Contact persons for Rotor-Gene Q (room 442):

Responsible person: Jelena Radosavljevic, phone: +318 11 3336 663, +381 65 883 8446
mail: jradosavljevic@gmail.com
Contact person: Jana Ognjenovic, phone: +318 11 3336 663
(if responsible person is not available)



This web page is supported by Faculty of Chemistry, University of Belgrade